Why You Should Use Commercially-Produced Lentivirus

You might know that lentiviral vectors are excellent for delivering genes to cells, but did you also know that they are great for screening cancer cells for potentially important therapeutic targets? In a recent paper (Zhao, et al., 2018. Molecular Cell 69, 699), researchers led by Prashant Mali of the University of California, San Diego used CRISPR delivered by lentivirus to show that mutations in the human KEAP1-NRF2 pathway can mask mutations in oxidative phosphorylation genes that might be used as targets for anticancer drugs.